FastQCFastQC Report
Sun 14 Apr 2019
SRR6576373_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576373_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences337065
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG12170.36105795618055864No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA6520.1934345007639476No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5630.16703009805230445No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA5330.15812973758770565No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT5050.1498227344874134No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA4790.14210908875142778No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG4700.13943898061204812No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG4600.1364721937905152No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG4360.12935190541883615No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG4350.12905522673668282No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA4150.12312165309361696No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC3980.11807811549701096No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT3760.11155118448963848No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT3720.11036446976102532No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT3680.10917775503241214No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC3640.10799104030379895No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG3610.10710100425733908No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC3590.10650764689303249No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG3530.10472757480011274No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA9100.039.3624081
TACATGG21050.038.8433042
GTACATG21250.038.592221
ACATGGG21350.037.85733
CCTATAA550.00267741234.2773131
TCAACGC10600.034.135314
AACGCAG10500.034.0128756
ATCAACG10750.033.6590083
CAACGCA10650.033.533825
ATGGGAG3250.033.25555
CATGGGG7650.033.170454
CATGGGT6050.032.6221774
ATGGGTA4650.032.3382155
GTATTAT902.5396292E-531.4208681
TAGTACT600.00416054231.3276464
CATGGGA9700.029.5513364
ACGCAGA12100.029.5153037
GGTACCT4550.028.9307048
ACCGGGG650.006140165528.9307028
TATACCT650.006153536528.9178284
Produced by FastQC (version 0.11.2)