FastQCFastQC Report
Sun 14 Apr 2019
SRR6576374_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576374_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences180728
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG3740.20694081713956886No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1860.10291709087689788No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAC451.6084958E-552.2222251
ATGGGTA1300.043.3846175
GGGTACC1209.640644E-1139.1666687
TACATGG15000.038.2266652
GTACATG15200.037.7236821
ACATGGG14600.037.6643833
GTGTTAA500.001698234437.61
ATGGGAG3550.037.0704235
CATGGGG3400.035.9411774
GGTACCT1353.4197E-1034.8148168
CATGGGT2200.034.181824
ATGGGAC1107.635208E-834.181825
ATGGGAT2150.032.79075
CATGGGA9050.032.718234
TGGGAGT1309.096766E-932.5384646
GTATCAA7900.030.9367081
TAGGCCA650.00614063928.9230774
TGGGTAC1652.928573E-928.484856
AACGCAG8600.027.8720956
AGTATAG856.793723E-427.647062
Produced by FastQC (version 0.11.2)