FastQCFastQC Report
Sun 14 Apr 2019
SRR6576374_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576374_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences180728
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG3150.17429507325926252No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2300.127263069363906No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA1970.1090035854986499No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAACGC555.2663774E-542.7258873
TACATGG15050.042.1581042
ACATGGG15200.041.432873
ATGGGTA1253.6379788E-1241.358665
ATGGGAG3300.041.3016935
GTACATG15650.041.2680441
GGGTACC1357.2759576E-1238.2950557
GGTACCT1401.2732926E-1136.9273768
CATGGGG4300.036.06864
CATGGGA10250.034.847654
TGGGTAC1502.7284841E-1134.465556
CATGGGT2200.034.180714
ATAGACT550.00271141234.180714
GAAGTTA550.00271141234.180716
GAGTATT851.7109425E-533.276681
GAGTAGC600.004090925731.4279771
GTATAAG753.2074435E-431.4279751
ACGCCGC600.004152450731.3323216
CGCGGTA600.004152450731.33232194
AACGCCG600.004152450731.3323215
Produced by FastQC (version 0.11.2)