FastQCFastQC Report
Sun 14 Apr 2019
SRR6576375_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576375_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences163989
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG3150.1920860545524395No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA2600.15854721963058496No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2520.1536688436419516No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1850.11281244473714701No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTGA250.005212759656.441768
CATGGGC1700.038.69914
CCAATCC500.001699236537.5934143
TATACTC801.1539943E-535.2438245
CTCATGC550.002700426834.2071278
CCTACTC550.002712580434.175833
ATATACT1001.4033267E-632.8942384
GTATCAA9100.032.5625531
TACATGG15950.030.64042
TATATAC1102.9520634E-629.903853
ACATGGG16700.029.5457213
GTACATG16650.029.0966011
AACGCAG10050.028.9899446
GTATTAA650.006116826628.9444921
TATACTG650.00614425728.918015
ATGGGAG3950.028.551965
ATGGGCA1354.6010246E-727.8469725
ATCAACG10550.027.6160163
CAACGCA10600.027.4857485
TCAACGC10650.027.356714
Produced by FastQC (version 0.11.2)