FastQCFastQC Report
Sun 14 Apr 2019
SRR6576376_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576376_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences535159
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG17100.3195312047447581No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA10170.19003697966398772No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT9330.17434070995722767No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA9110.17022978217688575No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA8530.15939188166507523No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA6890.12874678366616277No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG6860.12818620260520705No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG6790.1268781801296437No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT6770.1265044594223399No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG6600.12332783341025752No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC6300.11772202280070035No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC6150.11491911749592178No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG6090.11379795537401034No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG6070.11342423466670652No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT5760.10763156370349747No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT5650.10557609981332651No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG5390.10071773061837697No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12400.042.839971
GTACGGT555.2725194E-542.7365761
GTACATG29400.037.8960081
TACATGG29500.037.4488262
AACGCAG14500.037.2735256
TCAACGC14700.036.4466974
ACATGGG30300.035.829443
ATCAACG14850.035.762073
CAACGCA15350.034.903355
TACGGTG550.002712819334.189262
GGTACCT6700.030.8637398
TGGGTAC7150.029.5785686
GGGTACC7050.029.33157
CATGGGG17000.029.3040474
ACGCAGA18600.028.804657
CGCAGAG18600.028.5519758
ATGGGTA6750.028.5463625
TATCAAC19600.027.102842
ATGGGGG6350.025.1637785
GCAGAGT22050.024.0846589