FastQCFastQC Report
Sun 14 Apr 2019
SRR6576377_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576377_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences479171
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG15710.3278579045893846No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA9360.19533736390557857No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA8030.1675810931796791No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA7790.16257244282312577No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT7490.15631162987743413No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA7110.14838126681289143No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG6990.1458769416346148No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG6510.13585964092150818No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG6190.12918144044610377No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC6080.12688580903268354No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG6020.12563364644354522No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT6010.12542495267868883No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG5710.11916413973299719No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC5610.11707720208443333No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT5360.11185985796302364No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG5140.10726859513618311No Hit
GGGCGGGGACGGGCGGTGACTCGCCTCGCGGCGGACCGCCCGCCCGCTCC5110.10664251384161397No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT5060.10559904501733203No Hit
AGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGG5040.10518165748761923No Hit
GGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT4910.1024686385444862No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA4850.10121647595534788No Hit
GATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGG4820.10059039466077872No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTAGG250.005237517456.3909232
CTATAGT405.677889E-446.9924354
GTATCAA11600.043.040171
GTACATG24500.037.4882051
TACATGG24700.037.099292
ATGGGTA5750.034.324915
GGGTACC5500.034.176327
ACATGGG26650.034.0320473
GGTACCT5550.033.8931778
AACGCAG14900.033.7462436
GTAGGAC702.1851735E-433.5660253
TCAACGC15000.033.521274
TGGGTAC5650.033.2689826
GTACTAT851.7272016E-533.247461
CAACGCA15150.033.1893775
ATCAACG15150.033.1893773
GTATATA1001.3855515E-632.9703981
ATGGGCG1001.4106627E-632.8947075
GTACTAA600.00411529931.4003771
GACGTGT600.004161860331.328296
Produced by FastQC (version 0.11.2)