FastQCFastQC Report
Sun 14 Apr 2019
SRR6576378_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576378_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences253036
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8370.33078297159297493No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4230.16716988886956796No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3970.1568946711139917No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3710.1466194533584154No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3690.14582905199260185No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA3150.12448821511563572No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG3070.12132660965238147No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG2980.11776980350622047No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG2770.10947058916517807No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG2730.10788978643355095No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG2670.10551858233611028No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC2660.10512338165320351No Hit
GGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT2630.10393777960448315No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC2610.1031473782386696No Hit
AGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGG2580.10196177618994925No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT2570.10156657550704248No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGTAG250.00523142256.399766
CGTAGCT250.00523142256.399768
GTATCAA7050.042.6749231
ACTAGGC450.001012476441.77763
GTACATG15100.041.4053571
TACATGG15100.041.094042
CATGGGT4300.038.2556534
ATGGGTA2250.037.5998425
ACATGGG16700.037.1495443
CCGAACT651.4087724E-436.160841
ATCAACG8700.034.5745663
AACGCAG8850.034.519636
TCAACGC8850.033.988564
CAACGCA9000.033.94435
TGCCCGC702.1777317E-433.5779231
TACTAGG702.1777317E-433.5779232
GTAGAAC702.1802621E-433.571293
GTGTAAG851.7470875E-533.1828881
ACGCAGA9350.032.673667
CGCAGAG9650.031.6578968
Produced by FastQC (version 0.11.2)