FastQCFastQC Report
Sun 14 Apr 2019
SRR6576378_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576378_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences253036
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7320.289286899887763No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4270.16875069160119507No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3930.15531386838236455No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3840.15175706223620353No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3430.13555383423702555No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG3290.13002102467633064No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG3290.13002102467633064No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA3130.12369781374982217No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3120.12330261306691538No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT3110.1229074123840086No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG2920.11539859940877976No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG2770.10947058916517807No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG2700.10670418438483062No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT2690.10630898370192383No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATGCG156.8966317E-493.997634
GTATCAA6450.042.3629651
TTAGCCC450.001012581341.7767224
GTACATG13700.040.233091
TACATGG14200.039.386332
ACATGGG14500.038.247313
TACCGTG500.001699730537.5990527
ATGGGTA3250.037.599055
CATGGGG6300.035.062614
CATGGGT4450.034.8530544
TGGGTAC3250.034.7068146
GTCCTAA550.002681943834.2622071
ATCAACG8100.034.2337043
GGTACCT3550.033.0977558
TCAACGC8450.032.815744
AACGCAG8650.032.6003346
CAACGCA8650.032.0569955
GGGTACC3700.031.7559557
TATGCAC600.004155419831.3325444
CTTATCC600.004155419831.3325443
Produced by FastQC (version 0.11.2)