FastQCFastQC Report
Sun 14 Apr 2019
SRR6576379_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576379_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences229299
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7080.3087671555479963No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4420.19276141631668695No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4120.17967806226804303No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3620.15787247218696984No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3450.15045857155940495No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3340.14566134174156886No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA3010.13126965228806056No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2860.1247279752637386No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG2820.12298352805725275No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG2800.12211130445400983No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT2800.12211130445400983No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC2740.11949463364428106No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG2690.11731407463617372No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG2680.11687796283455228No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT2640.11513351562806641No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC2480.10815572680212299No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC2470.10771961500050152No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG2350.10248627338104396No Hit
GGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT2320.10117793797617958No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA7350.048.6882321
GGCTTAG405.615915E-447.086651
GTACATG15100.040.5381741
TACATGG15450.039.2378922
ACATGGG15350.038.575063
AACGCAG9200.037.79976
ATCAACG9250.037.5953753
TCAACGC9400.036.995454
CAACGCA9450.036.799715
CATGGGT3800.034.627324
GTACTAG550.002688098534.2448351
CATGGGG7000.033.56734
ACGCAGA10400.033.4381947
CTATTAA851.7267736E-533.2376331
CGCAGAG11000.031.628098
ATACTAG600.004116805331.3910981
CATGGGA4950.031.3294814
ATGGGTA2600.030.7269885
ATGGGAG2150.030.6008875
ATGGGCG650.00614772928.919525
Produced by FastQC (version 0.11.2)