FastQCFastQC Report
Sun 14 Apr 2019
SRR6576386_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576386_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences268569
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5800.215959399632869No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3210.11952235738301889No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3000.11170313774113916No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2980.11095845015619824No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2880.10723501223149359No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGG555.1701893E-542.8689961
GTATCAA8250.041.1542321
AACGCAG9000.037.605296
AGTGCGG500.001700086637.59828694
CAACGCA9200.036.276845
TCAACGC9250.036.080754
CTATACC801.15423645E-535.254964
ATCAACG9350.035.1921163
CATGGGT4150.035.113374
GTATAAG959.1740003E-734.746451
AGCGTCC550.002713925234.1802648
ACATGGG15450.034.075993
GTACATG15600.033.8555151
TACATGG15600.033.7483372
ACGCAGA10750.031.4776367
CCCTATA600.004152497331.3377422
CGCAGAG10950.030.9027028
TATAAGA953.7381018E-529.6883852
CGACGGT804.772516E-429.3736637
ACCGGCG650.006147256628.9217598