FastQCFastQC Report
Sun 14 Apr 2019
SRR6576387_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576387_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences243438
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6150.252631060064575No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3460.14213064517454135No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3300.13555812979074755No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2820.11584058363936608No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2820.11584058363936608No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT2600.10680337498664959No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT2520.10351711729475266No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA2450.10064164181434287No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACCGT352.9369575E-453.6992656
GTAATAC500.001701729737.589483
CATGGGT3350.035.0648164
GTATCAA8150.034.5986561
ACATGGG14700.034.520953
GTACATG15000.033.8374821
TACATGG15150.033.502462
AACGCAG8400.031.8839386
GGGCAAT600.00415610931.3310031
CATGGGG6000.030.5414544
TATAAGG1102.9575021E-629.9068682
ATGGGTA2101.8189894E-1229.0871015
ATGGGGC2101.8189894E-1229.0871015
GGTACCT2101.8189894E-1229.0871018
TCAACGC9250.028.954064
ATCAACG9300.028.7983953
CGCAGAG9400.028.492038
CAACGCA9450.028.3412765
CTACACT1005.3166787E-528.1921124
ACGCAGA9600.027.8984457