FastQCFastQC Report
Sun 14 Apr 2019
SRR6576388_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576388_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences163900
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4630.28248932275777916No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2470.15070164734594266No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2450.14948139109212935No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA2010.12263575350823673No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1930.11775472849298353No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC1850.11287370347773033No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT1830.11165344722391703No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG1770.1079926784624771No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG1670.10189139719341062No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG1670.10189139719341062No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGCAT405.662583E-446.99975
GTGTAAT608.775535E-539.1783641
ATACCAA608.7913344E-539.1664166
CATGGGG5400.038.2960474
TAATACC500.001697827637.599764
TGGGGGG906.1475293E-736.5553176
GTAATAC651.4082638E-436.153613
GTACATG9900.035.1418041
CACGAGC550.002710334534.181694
TACATGG10500.033.5814552
GTATAAG702.1730538E-433.5814551
ATACTAC702.176954E-433.5712133
ACATGGG10400.033.442093
GTATCAA6150.032.87161
AACGCAG6050.031.8510326
GCAACTA300.00415681531.33313250-51
CGCAGAG6500.030.3690368
ATCAACG6600.029.9088953
CAACGCA6650.029.6840175
ACGCAGA6700.029.4624967