FastQCFastQC Report
Sun 14 Apr 2019
SRR6576388_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576388_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences163900
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG3790.2312385600976205No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2540.1549725442342892No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2430.14826113483831604No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2250.13727882855399634No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA2040.12446613788895669No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG1920.11714460036607688No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG1740.10616229408175717No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT1740.10616229408175717No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG1690.10311165344722391No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG9400.040.129731
GTATCAA4250.039.9408871
CATGGGT2500.037.5953644
TACATGG9800.037.4035532
ACATGGG9700.037.304683
TTATACC801.153582E-535.2456554
CTATTCT550.00271188834.1776054
CATGGGG3750.033.8358274
ATGGGTA1554.1836756E-1133.3507275
TAGGACC851.7471348E-533.172384
CTAGGAC753.265759E-431.329473
ATCAACG5550.030.4827253
AACGCAG5750.030.2397486
CAACGCA5800.029.9790615
GAAATTC804.7581512E-429.3803494
TCAACGC5950.029.2232884
ATGGGGG1452.6340786E-829.1688165
CCCCTGT650.006142695428.919518
GGTACCT1303.3058132E-728.919518
TAGCACT650.006142695428.919514