FastQCFastQC Report
Sun 14 Apr 2019
SRR6576389_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576389_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences146566
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4360.2974769046027046No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2530.17261847904698224No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2410.16443104130562342No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2090.14259787399533316No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG2030.13850415512465375No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA1950.13304586329708118No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG1870.1275875714695086No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT1720.11735327429281006No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT1600.10916583655145122No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC1540.1050721176807718No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC1510.10302525824543209No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA1480.10097839881009238No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAACGC250.005225824756.4030722
GTATAAG250.005225824756.4030721
TGCCCGC352.928253E-453.717211
GCAAACG451.6064698E-552.2250631
GTATCAA5300.047.002561
AACGCAG5700.041.2162486
ATCAACG6150.039.7284433
CAACGCA6250.039.092795
TCAACGC6350.037.737214
AGCGTAG250.001702355737.5892236-37
ATAAGCC500.00169957937.589223
TATCAAC6500.036.878932
CCTAGTA651.4070286E-436.155822
GTACTGT801.1540409E-535.239896
ACGCAGA6700.035.0645687
CGCAGAG6850.034.296738
GTACACT550.002713127534.172024
GTATAAT702.1750508E-433.5732571
ATGGGGC1151.1286829E-732.686285
ACATGGG9650.032.622773