FastQCFastQC Report
Sun 14 Apr 2019
SRR6576389_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576389_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences146566
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG3900.26609172659416236No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2710.1848996356590205No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2410.16443104130562342No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2400.16374875482717685No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2380.1623841818702837No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA1910.1303167173832949No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT1900.12963443090484833No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA1820.12417613907727577No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG1690.11530641485747037No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG1600.10916583655145122No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG1590.10848355007300466No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC1560.10643669063766495No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT1480.10097839881009238No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATGAG250.005230110656.39143
TTACACC555.2652096E-542.7207574
GTATCAA5850.039.455751
GTACATG9950.035.033111
TACATGG10100.033.976712
AACGCAG6300.033.566316
ACATGGG9800.033.566313
CATGGGG4100.033.238844
CAACGCA7000.032.223665
ATCAACG7000.032.223663
TCAACGC7050.031.9951214
ATAATAG600.004152350631.3285583
ATGGGGT1102.94872E-629.9045315
CTAGTAC804.7650415E-429.3705223
TATACAG804.7650415E-429.3705225
CCTTGAA650.006131238328.9285372
TAAGAGT650.00614147528.9186696
ACGCAGA7650.027.6428437
CTGTGCG700.00878857426.8713849
GTGCACT700.00881789626.8530486