FastQCFastQC Report
Sun 14 Apr 2019
SRR6576390_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576390_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences246958
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5560.22513949740441694No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3310.134030887843277No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2850.11540423877744393No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2710.109735258626973No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA7300.046.3806421
TACATGG15900.044.0672842
GTACATG16150.043.3851281
GTACTAG555.2546067E-542.7498321
ACATGGG16500.042.439043
CATGGGT4900.042.2007184
AACGCAG8700.037.272896
ATCAACG9000.036.030463
ATACTGG1055.0878953E-835.806676
CATGGGA7350.035.8066674
CAACGCA9100.035.118085
TCAACGC9200.034.7363624
ATGGGTG2050.034.38755
GGTACCT2200.034.1790928
CATGGGG6050.032.62554
ACGCAGA10100.032.106357
CGCAGAG10100.032.106358
TATCAAC10550.032.0927662
ATGGGGG2900.030.790655
GGGTACC2500.030.0776047
Produced by FastQC (version 0.11.2)