FastQCFastQC Report
Sun 14 Apr 2019
SRR6576392_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576392_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences385417
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG10270.26646463440896484No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6580.17072417667098233No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT6350.16475661426454982No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5680.14737284551537685No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG5570.14451879392969172No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA5460.14166474234400664No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA5450.14140528310894435No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC5330.13829177228819695No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5270.13673501687782322No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA5170.1341404245272004No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC4590.11909178889358799No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT4550.11805395195333886No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG4530.11753503348321429No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA4430.11494044113259144No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG4380.11364314495728005No Hit
ACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACG4160.1079350417859098No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCG4140.10741612331578523No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG4080.10585936790541155No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC4050.1050809902002247No Hit
CGGCCGGTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCC3970.10300531631972643No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT3930.10196747937947728No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTAAA450.001013212441.776975
GTATCAA16400.039.8656271
ATATAAC608.814171E-539.165913
CCGCTAA500.001700785537.5992744
CAACGCA19000.034.8782735
TCAACGC19250.034.425314
AACGCAG19450.034.312966
CCCGCTA550.002715035834.1811563
ATCAACG19150.033.8687973
ATGGGTA3800.032.1572725
AACTCGA452.5931666E-531.33272736-37
CATGGGT5950.030.8061284
GTACATG27300.030.6679691
ACATGGG27350.030.244393
TACATGG28300.029.7311972
ACGCAGA22550.029.595887
TGAACCG804.7755954E-429.3744345
GGTACTC650.006149742828.922523
CTTACCC650.006149742828.922523
CATGGGG10650.028.6848934
Produced by FastQC (version 0.11.2)