FastQCFastQC Report
Sun 14 Apr 2019
SRR6576393_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576393_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences279045
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8130.29135085738859323No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5650.20247630310523393No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5150.18455804619326632No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4880.1748821874608038No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA4350.15588883513411814No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4100.14692970667813435No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT3620.12972818004264544No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG3450.12363597269257647No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3400.1218441470013797No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3340.11969395617194359No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG3300.11826049561898619No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC3290.11790213048074683No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG3220.11539357451307136No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA3160.11324338368363526No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC3060.10965973230124174No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC2890.10356752495117275No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG2830.10141733412173663No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG2810.10070060384525795No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTAAA459.976985E-441.9040871
GTATCAA8250.039.999361
CATGGGG8150.035.7534454
GTACATG18350.035.4529151
TACATGG18500.035.312372
ACATGGG18850.034.1580433
ACCTACT851.750432E-533.1753968
AACGCAG10100.032.1078726
CGAATAA300.004159823531.33231716-17
CAGTCGA300.004159823531.33231720-21
ATACCTA600.004156291531.3323176
TAACACT753.2698584E-431.3323154
TCAACGC10350.030.4241354
ATCAACG10750.029.7292693
ATGGGGG2700.029.5916355
CAACGCA10800.029.5916355
CTTGTAT804.772621E-429.3740463
GATATAG650.006056278529.0105251
GGTACCT3300.028.4839258
ACTGGTA856.8043533E-427.6461648
Produced by FastQC (version 0.11.2)