FastQCFastQC Report
Sun 14 Apr 2019
SRR6576395_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576395_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences398268
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8560.2149306497132584No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5090.1278033886729539No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4460.11198489459359025No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4160.10445227836532185No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA9650.040.926021
GTACATG21200.035.4839251
TACATGG21750.034.3704642
ACATGGG21700.034.0077863
TCAACGC12050.032.7624364
CAACGCA12200.032.7448545
ATCAACG12100.032.631153
AACGCAG12250.032.227546
TTCGTCG300.00416087531.33232760-61
CATGGGT5450.029.320164
CCACGCG650.006150368628.9221489
CTAATGT1154.1883923E-628.6077774
CATGGGG8800.028.3059124
ACGCAGA14050.028.0987437
CGCAGAG14100.027.9991048
TAGATTG700.00883059426.8562835
ATGGGTA3150.026.8562815
TATCAAC14300.026.6315562
CATGGGA10450.026.0852894
ATGGGGG3850.025.6355425
Produced by FastQC (version 0.11.2)