FastQCFastQC Report
Sun 14 Apr 2019
SRR6576395_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576395_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences398268
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7420.1863067080458385No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4710.11826207478381393No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4170.10470336557293078No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4110.1031968423272771No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4040.10143923187401448No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATGG23550.037.7250372
GTACATG24000.037.6857261
ACATGGG23900.037.1725773
GTATCAA10800.035.766551
ATGGGTA3450.034.058475
GGGTACC3300.032.7539447
CATGGGG10150.031.488154
GAGTAAT753.228314E-431.4047721
CATGGGT5150.031.0295854
CATGGGA12450.030.2012444
TGGGTAC3650.029.6168756
ATGGGAG5700.028.8600735
ATCAACG13250.028.0265733
GGTACCT3900.027.7148768
AACGCAG13400.027.7093626
TCAACGC13400.027.7093624
GTCCTAT1554.955109E-827.3525431
GTACTAG700.00873167426.9183791
GTAAACC700.00882317326.860913
CGCCTAA350.00883919726.85416622-23
Produced by FastQC (version 0.11.2)