FastQCFastQC Report
Sun 14 Apr 2019
SRR6576396_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576396_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences358587
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7760.21640494496454138No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4690.1307911329747035No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4080.11377991951743928No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3880.10820247248227069No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT3640.10150953604006838No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA3630.10123066368830996No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTTA250.00523855256.3851994
GCAAACG555.2796306E-542.722011
GTACATG23700.037.6746861
ACATGGG22950.036.443593
TACATGG24200.036.119522
CATGGGG7750.032.7397884
TAGGCCC1052.0643547E-631.3251084
CATGGGT5050.030.7048074
CAAACGC804.7778428E-429.3713842
GTATCAA12850.029.2570971
GTAATAG1005.3179243E-528.1965271
ATGGGTA3050.027.7304215
AACGCAG13250.027.660666
TCTACAC856.8173377E-427.63983
ATGGGCG1057.4208336E-526.8500925
CATGGGA12450.026.4187664
CATGGGC4950.025.6296334
CAACGCA14350.025.540335
ATCAACG14350.025.540333
TCAACGC14350.025.540334
Produced by FastQC (version 0.11.2)