FastQCFastQC Report
Sun 14 Apr 2019
SRR6576396_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576396_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences358587
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7330.20441343383892893No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4230.11796300479381572No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4210.11740526009029886No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4050.112943302462164No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4040.11266443011040557No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3610.10067291898479308No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGGGT5750.039.2295464
GTACATG22300.037.963921
ACATGGG21800.037.724323
GTATCAA12350.037.702631
TACATGG22200.037.6796532
AACGCAG13400.033.3164446
ATCAACG13700.033.2729263
GTATAGG1001.3998597E-632.9231531
TCAACGC13800.032.6912884
CAACGCA14000.032.5599375
ATGGGTA3500.032.2242665
ATGGGAC2050.032.0932775
CGCAGAG15300.029.1994698
CATGGGG8450.028.9192144
ACGCAGA15550.028.7099917
GGGTACC3450.028.6048767
TATCAAC16400.028.3681642
TGGGTAC3700.027.9422156
CATGGGA11400.027.6191184
TAAGGGT1057.414316E-526.8535584
Produced by FastQC (version 0.11.2)