FastQCFastQC Report
Sun 14 Apr 2019
SRR6576397_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576397_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences234432
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5470.23332992082992082No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3320.14161889161889163No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2870.12242355992355992No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2870.12242355992355992No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA2680.11431886431886433No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA2640.11261261261261261No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA2480.10578760578760578No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG2360.10066885066885066No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTAGA200.002159445570.511362
TAATACC652.776398E-643.3823474
GTACATG16100.043.2119331
TACATGG16100.043.2119332
ACATGGG16050.043.044483
GTATCAA6350.041.45551
CATGGGT3600.039.1646234
ATGGGTA2550.038.703865
GGTACCT2350.035.998128
CATGGGG5650.034.936234
CATGGGA8900.034.3240474
TACTATC702.1803894E-433.5696757
GGGTACC2700.033.072357
TGGGTAC2850.032.9807366
ATGGGAG3350.032.2669755
AACGCAG8200.032.0958866
ATGGGAC2200.032.043785
ATCAACG8250.031.9013653
CAACGCA8350.031.5193145
TCCTATA753.2645653E-431.3383832
Produced by FastQC (version 0.11.2)