FastQCFastQC Report
Sun 14 Apr 2019
SRR6576397_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576397_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences234432
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5230.22309241059241058No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3640.15526890526890527No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3150.13436732186732187No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3040.12967512967512967No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG2540.10834698334698334No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT2520.1074938574938575No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA2510.10706729456729457No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTAGGC952.0982043E-839.5926933
GTACATG17350.036.9805641
TACATGG17800.036.186722
CTATCCC651.407181E-436.1664054
ACATGGG18100.035.586943
GTATCAA7350.035.3028221
GTACTAG959.134692E-734.76231
CATGGGT4500.033.433834
ATGGGAG3400.033.1879925
AATGCGA501.4096768E-632.8973920-21
GGGTACC2500.031.957467
GTATTGA753.1962155E-431.4516071
TACTAGG1052.0505395E-631.3442172
GGTACCT2550.031.3308458
TGCGACG552.9653402E-629.90671322-23
ACGAGTC552.9653402E-629.90671326-27
ATGGGTG1901.4551915E-1129.694525
ACACCTA953.7308277E-529.694526
CATCGTC1154.1802705E-628.6064249
CATGGGA9900.028.4947414
Produced by FastQC (version 0.11.2)