FastQCFastQC Report
Sun 14 Apr 2019
SRR6576398_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576398_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences213636
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5360.2508940440749686No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3120.14604280177498175No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2960.1385534273249827No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2720.12731936564998408No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA2540.11889381939373515No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG2460.11514913216873561No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT2380.11140444494373608No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG2210.10344698459061206No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA2200.10297889868748714No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTAAA200.002163082670.479295
CCCGCTA352.9363472E-453.69853
GTATCAA7950.043.7663041
GTACATG15400.039.386221
AACGCAG9000.038.633096
TACATGG15850.038.2679982
ACTATAC500.001701381537.588953
ACATGGG16200.037.414933
ATCAACG9350.036.6844023
CAACGCA9600.035.729085
TCAACGC9600.035.729084
GTTATGT550.002706649734.195791
TCTAGAC550.002715992534.1717763
ACGCAGA10250.033.4633337
CATGGGT3400.033.166724
CGCAGAG10400.032.980698
ATGGGAC2000.032.890335
ATGGGAT2800.031.8834865
TATCAAC10950.031.7755372
TGCCCGC600.004145166431.346141