FastQCFastQC Report
Sun 14 Apr 2019
SRR6576398_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576398_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences213636
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4810.22514931940309685No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3850.18021307270310247No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3180.1488513171937314No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3010.1408938568406074No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA2650.1240427643281095No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG2210.10344698459061206No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG2190.10251081278436218No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCGTG200.002162505670.4840247
GCATTAT405.6269264E-447.066471
GTATCAA6450.043.782761
ATGGGAG3050.043.1377645
GTACATG15250.041.356771
TACATGG15300.040.846952
ACATGGG15350.040.407783
CATGGGA8650.038.0260624
GTATAAG1003.2643584E-837.6531751
TAGACTA500.001700819637.591485
CATGGGG5750.033.5054474
ATCAACG8400.033.0044253
AACGCAG8400.032.4450266
TCAACGC8700.032.406454
TAGCGCG600.0041580831.3262334
TAACAGT600.0041580831.3262334
TGGGAGT1501.0622898E-931.3262316
CAACGCA8750.031.1472245
TATCAAC9300.029.3051852
ATGGGAT2900.029.1658045
Produced by FastQC (version 0.11.2)