FastQCFastQC Report
Sun 14 Apr 2019
SRR6576399_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576399_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences455346
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG12870.28264221053879907No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA8810.19347924435484223No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA7840.17217676228626141No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA7350.1614157146433701No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA7250.15921958247135146No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT6460.1418701383124042No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC6330.13901516648877996No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA6260.13747787396836691No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG5760.12649721310827372No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA5690.12495992058786067No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG5460.11990881659221778No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG5360.11771268442019914No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG5270.11573616546538237No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC5150.11310080685895998No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA4970.10914776894932646No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC4940.10848892929772086No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT4850.10651241034290407No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC4700.10321821208487611No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA11700.045.1472131
GTACATG25800.038.022661
TACATGG26600.036.576852
ACATGGG26600.035.8700523
ATCAACG14850.035.1329153
AACGCAG15050.034.6622246
CATGGGG12900.034.610184
TCAACGC15050.034.3499534
CCCTTAT550.002669659734.300161
CTATATT550.002716148534.1796234
CAACGCA15500.033.9591065
TGGGTAC5250.031.3313226
CATGGGT8100.030.1709024
GGGTACC5300.030.1490067
ATGGGTA6100.030.0472495
ACGCAGA17500.029.8095137
GGTACCT5500.029.052688
TTACACA1005.3189025E-528.1981874
TAGGTAT1853.274181E-1027.944155
CGCAGAG18850.027.9239338
Produced by FastQC (version 0.11.2)