FastQCFastQC Report
Sun 14 Apr 2019
SRR6576401_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576401_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences606448
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG16350.269602669973353No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA8590.14164446086061788No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA8340.13752209587631586No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT7870.12977204970582804No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA7560.12466031712529352No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA7200.11872411154789858No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG6210.10239954621006253No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA13700.046.6665531
GTACATG34700.037.661941
GTAATAC906.185837E-736.554053
TACATGG36200.036.2312242
TACGCTC651.4130447E-436.152364
ACATGGG36450.035.9738243
TCAACGC17700.035.5804564
CATGGGG17100.034.6301544
ATCAACG18300.034.413883
CAACGCA18500.034.2958835
AACGCAG18550.033.9500856
ATGGGGA9800.030.213045
GTATTAC953.744111E-529.6903321
CATGGGT9200.028.6075134
ACGCAGA22250.028.5156787
CGCAGAG22550.028.3447288
TATCAAC22900.028.3289832
ATGGGAG6100.026.1956425
GCAGAGT25550.025.0165829
GTTTAGG1701.2393684E-724.8874821
Produced by FastQC (version 0.11.2)