FastQCFastQC Report
Sun 14 Apr 2019
SRR6576402_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576402_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences532753
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG15230.2858735661741933No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA8430.15823467911020678No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA7650.14359374794698482No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA7370.13833802906787948No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT7070.1327069016974095No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA6650.1248233233787515No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA5670.10642830730188287No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG5580.10473896909074186No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA14550.040.382971
GTACATG32100.039.830441
ACATGGG31550.039.467053
TACATGG32700.038.671992
GTATAGG1101.7898856E-938.459271
CATGGGG14800.037.4635054
GGTACCT4100.035.5276078
TAGATCG550.00271907234.1731835
CATGGGT7350.033.24334
TCAACGC17800.032.7333034
CAACGCA17800.032.4693265
ATGGGTA5000.031.9519275
AACGCAG18100.031.9311586
ATCAACG18650.031.2414363
GGGTACC4850.030.0336487
TATAACG804.771419E-429.3813672
GTCTATA650.00614755128.926631
ATGGGGC5250.028.6403835
ACGCAGA20400.028.1007427
ATGGGAG4750.027.6982635