FastQCFastQC Report
Sun 14 Apr 2019
SRR6576402_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576402_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences532753
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG15230.2858735661741933No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT7310.1372118035937855No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA7250.1360855781196915No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA7150.13420853566286817No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA6900.12951592952080984No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA6310.11844137902555218No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA5680.10661601154756518No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG5620.1054897860734712No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5430.10192340540550687No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT5340.10023406719436587No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG504.561316E-756.3958322
TAACGCA503.0102168E-546.9921154
GTATCAA13550.041.679231
GTACATG33700.036.449231
TACATGG34000.036.3531952
ACATGGG35050.035.2608453
CATGGGT10150.033.334314
TCAACGC16800.033.286084
CATGGGG15800.033.013454
CAACGCA17450.031.7769015
ATCAACG17650.031.683073
AACGCAG17450.031.5076056
ATGGGTA6900.028.6038955
ATAGTAC1005.324329E-528.195273
GGTACCT6350.028.1397578
CTATATC1205.8430833E-627.412073
GCGGTAC700.0087745126.8930281
TATCAAC21150.026.8869042
ACGCAGA20600.026.6871917
CGCAGAG21300.025.8295428