FastQCFastQC Report
Sun 14 Apr 2019
SRR6576403_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576403_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences560338
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG11320.202020923085709No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA6820.12171225224775045No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6330.11296753031206164No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA6070.10832747377475738No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT6070.10832747377475738No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10450.052.6637571
TCAACGC13300.040.9920584
CAACGCA13450.040.8843355
ATCAACG13350.040.8385243
AACGCAG13850.039.7035566
GGTATCA4100.037.859281
TACATGG24150.036.788792
GTACATG24350.036.123091
GTACTAG550.002705284634.2089351
ATATACT1651.8189894E-1234.181464
ACATGGG25100.033.8920753
TATCAAC17400.032.149082
ACGCAGA17150.032.0638057
TATACTC902.5917552E-531.3330065
TATAACC753.2736163E-431.3330063
CGCAGAG18000.030.5496838
GGGTACC5000.030.0796877
CCTAGAC1102.9651073E-629.9087813
ATAACGC804.7780847E-429.3746933
GGTACCT5300.029.2638458
Produced by FastQC (version 0.11.2)