FastQCFastQC Report
Sun 14 Apr 2019
SRR6576405_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576405_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences295047
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5260.17827668134229463No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3630.12303124586930218No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3620.12269231681732062No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3170.10744050947815093No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3060.10371228990635391No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAAACG652.7704828E-643.4013671
GTATCAA7750.043.074691
GTACATG18400.031.6861441
ATCAACG10600.031.4719373
GTCTTAC600.00414816631.3454321
TCAACGC10650.031.3241834
CATGGGG7500.031.324184
AACGCAG10800.031.3241796
ACATGGG18200.031.2381253
TACATGG18100.031.1722532
CAACGCA10850.030.7467755
ATGGGGG3700.029.2076845
CAGCTTA650.00615563528.9146299
TATCAAC12050.027.7036382
ACCTAAG856.7886495E-427.6577341
TAGTATA1205.837146E-627.4086594
GTGTTAT1057.3837655E-526.8675141
CTATACT700.00883815326.8492974
ACGCAGA12650.026.7431747
ATTAGCC1258.015673E-626.3123133
Produced by FastQC (version 0.11.2)