FastQCFastQC Report
Sun 14 Apr 2019
SRR6576405_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576405_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences295047
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4840.16404166115906957No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4420.1498066409758445No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3850.13048768501289623No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACCG250.005237086756.3866775
GTACTAG451.5891244E-552.342961
GTACATG17050.038.6816021
TACATGG17250.038.135922
GTATCAA7750.037.686931
ACATGGG17250.037.318723
CATGGGT4050.032.486154
GTCTATA600.004109290431.4057791
CCCTATA650.006153953328.9162452
TAGTAGG650.006153953328.9162454
CATGGGG8050.028.6019384
AACGCAG10250.027.9688646
TCAACGC10450.027.4289254
CATGGGA7750.027.2838754
ATCAACG10600.027.0407813
CATGGGC3500.026.85084
CAATACC700.00883574426.8507984
GGTATCA4750.025.7857971
CAACGCA10950.025.7473435
ACTAGGC950.001304083224.7309973
Produced by FastQC (version 0.11.2)