FastQCFastQC Report
Sun 14 Apr 2019
SRR6576406_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576406_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences207079
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4380.21151348036256692No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG3680.1777099561037092No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2310.1115516300542305No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2110.10189348026598544No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA7750.048.5161251
GTACATG16450.042.01
TACATGG16250.041.938462
ACATGGG16600.041.9036143
CATGGGG6500.039.0461544
AACGCAG9700.038.7628866
ATGGGGG2550.038.7058835
ATCAACG9700.037.793813
TCAACGC9900.037.5050544
CAACGCA10150.036.581285
TATCAAC11050.034.027152
ATAACGC702.1788456E-433.571433
CGCAGAG11300.033.2743348
ACGCAGA11350.033.1277547
CATGGGA7850.031.7324854
ACACCGT600.004153179531.3333346
GTCTAAC600.004153179531.3333341
CATGGGT3650.030.9041084
ATGGGTG1102.9529801E-629.909095
ATGGGGA3000.029.7666655
Produced by FastQC (version 0.11.2)