FastQCFastQC Report
Sun 14 Apr 2019
SRR6576406_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576406_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences207079
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4280.2066844054684444No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG3340.1612911014636926No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2500.12072687235306333No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2360.11396616750129177No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2110.10189348026598544No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA7950.046.8274731
CCTTACC704.6177247E-640.284582
AACGCAG9050.039.987826
TCAACGC9400.039.4988824
GTATAAG608.663588E-539.2698751
CAACGCA9450.038.7925575
ATCAACG9500.038.5883833
ATGGGTA1700.035.940165
GTACATG14850.035.223891
TACATGG15050.034.663472
TATCAAC10850.034.65342
ACATGGG15100.034.548693
GTTAGGG550.002712266334.180852
CGCAGAG10950.033.4785088
ACGCAGA10900.033.2008977
CATGGGT3250.031.8144864
ATGGGAT2550.031.3324495
GGTATCA3400.030.4919031
CATGGGA7400.030.4856244
ATGGGAG2650.030.150095
Produced by FastQC (version 0.11.2)