FastQCFastQC Report
Sun 14 Apr 2019
SRR6576407_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576407_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences191384
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3740.19541863478660704No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG3290.1719056974459725No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA2600.13585252685699956No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2330.12174476445261882No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA1920.10032186598670735No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAACGC405.6652585E-446.9992142
GCAAACG450.001011918441.777081
GTATCAA8150.040.9441031
GTACATG15950.040.0745661
ACATGGG16150.039.2770043
TACATGG16500.038.7387472
GTAATGT500.001700805637.5895463
ATGGGAG3350.036.4674725
CATGGGA8800.035.7741474
TGGGAGA1457.385097E-1032.4047856
GTTGGTC600.00415273631.3328111
AACGCAG10500.031.3246236
ATCAACG10600.031.0291063
TCAACGC10600.031.0291064
GGTATCA4200.030.2137811
CAACGCA10900.030.1750975
CATGGGG4400.029.900784
ATAAAGC1102.9578368E-629.900783
ATATGAT953.743228E-529.6759573
CATGGGC3500.029.5346454
Produced by FastQC (version 0.11.2)