FastQCFastQC Report
Sun 14 Apr 2019
SRR6576408_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576408_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences358545
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG10970.3059588057287091No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA6760.18853979277357097No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA6610.18435621749013373No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC6100.17013206152644716No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA6030.16817972639417644No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT5760.16064929088398947No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA5400.15060871020374011No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA5080.14168374959907404No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC4990.1391736044290117No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG4820.13443221910778286No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG4800.1338744090699912No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG4540.12662287857870003No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG4190.11686120291734649No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC4120.1149088677850758No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA4060.11323543767170091No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT4000.111562007558326No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG3780.10542609714261808No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC3760.10486828710482646No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG3640.10152142687807666No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC3630.10124252185918084No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3620.10096361684028504No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGGTA1101.7898856E-938.452495
TAGGTAT1500.037.5979925
CTTAGGT1650.037.033493
TTAGGTA1700.035.9392554
AGGTATA1700.035.9392556
GTACATG20550.034.171361
TACATGG20850.033.1395452
TAGTATG1151.1341399E-732.6939057
ACATGGG21500.032.137653
GTCTTAG2200.032.1333771
ATCCTAC600.004101810531.4192981
GTATATA600.004101810531.4192981
GTATACA600.004101810531.4192981
AACGTAG300.004161035631.3316692-93
TAGCACC600.00415828631.331664
TCTTAGG2450.030.696522
GGTATAG1859.094947E-1230.4848587
GGTAATC1252.3566281E-730.0783928
AATAGTA1252.3566281E-730.0783925
GTATCAA10700.029.9511091
Produced by FastQC (version 0.11.2)