FastQCFastQC Report
Sun 14 Apr 2019
SRR6576411_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576411_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences253886
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4400.17330612952269916No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2940.11580000472653079No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTAA250.00523821456.381334
TGCCCGC558.7678745E-751.286061
TACGCTA853.9496808E-738.6930669
GTCCTAT1101.7807906E-938.4645461
CCCGCTA500.001702296737.587553
TATACGC906.180999E-736.5434537
ATACGCT959.4365714E-734.6201138
CTGCGGG550.002717448934.17059
GCAAACG702.1768991E-433.580161
ACATGGG16700.032.917243
ATATACG1001.409071E-632.889116
GTATCAA9450.032.336451
TACATGG16800.032.1809842
GTACATG16850.032.085491
CAAACGC600.00414964631.341482
GTCTAAC753.263429E-431.3414781
CATGGGG6700.030.8554524
CATGGGT2850.029.6743814
CCTATTC1154.1902695E-628.5992263
AACGCAG10800.027.842636
Produced by FastQC (version 0.11.2)