FastQCFastQC Report
Sun 14 Apr 2019
SRR6576412_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576412_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences327726
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG9460.2886557673178204No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5760.17575657714066018No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4720.14402275071248544No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4700.14341248481963592No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4690.14310735187321116No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC3990.12174804562347814No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG3860.1177813173199563No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3770.11503512080213349No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA3760.11472998785570873No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC3740.11411972196285923No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG3480.10618626535581553No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC3430.10466060062369176No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG3370.1028298029451432No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT3330.10160927115944417No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAACG200.002159411870.5179752
ACGCCTA200.002162027970.496456
CTAACGC250.00523375156.3971673
GTATCAA6750.045.967271
GTACATG19300.041.6531071
TACATGG19350.041.545472
ACATGGG19850.040.01313
GTATATA608.797053E-539.176652
TTACACT608.81289E-539.1646964
ATCAACG8300.036.8053743
AACGCAG8400.036.3672186
CAACGCA8550.035.72925
TCAACGC8600.035.521474
ACGTAGG550.002714857734.180194
GATTTAT702.1783443E-433.5799871
TAGTATA702.1783443E-433.5799872
ATGGGTA3250.033.2598655
TGGGTAC3250.033.2598656
TATAATA851.7479206E-533.184932
CATGGGT5200.032.5368234
Produced by FastQC (version 0.11.2)