FastQCFastQC Report
Sun 14 Apr 2019
SRR6576412_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576412_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences327726
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG9180.2801120448179272No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA6160.18796189499765048No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA5280.16111019571227184No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4890.14921001080170632No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4390.13395336348046843No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA4150.12663017276627425No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG4060.12388397624845146No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3860.1177813173199563No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG3630.1107632595521869No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3520.10740679714151455No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT3430.10466060062369176No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG3420.104355467677267No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC3370.1028298029451432No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG3320.10130413821301941No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG3310.10099900526659465No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA7600.047.7813841
CTATATG405.6649826E-447.0096934
ATCAACG9300.038.9220053
TCAACGC9400.038.507944
CAACGCA9550.038.3953485
AACGCAG9700.037.801616
GTACATG17650.035.5376361
TACATGG17800.035.389322
ACATGGG18550.034.2119063
TATTAGT550.002711444434.1888664
TATTAGG753.2665193E-431.3397962
ACGCAGA12150.030.1698537
TATCAAC12250.029.9327012
CGCAGAG12550.029.2082638
ATGGGGG4350.029.178435
TTAGTGC650.006141645428.929046
GGTATCA3400.027.7416741
GCATTAT700.00868108326.9490571
GCATATA700.00868108326.9490571
ATATACT700.00881809926.862684
Produced by FastQC (version 0.11.2)