FastQCFastQC Report
Sun 14 Apr 2019
SRR6576414_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576414_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences414290
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7300.17620507374061647No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA9300.050.728181
ATCAACG12450.036.614573
CAACGCA12700.035.893815
TCAACGC12700.035.893814
TACGACG801.1577084E-535.2462435
TATCAAC13700.034.3111952
AACGCAG13300.033.5678526
GACGGTA702.1839661E-433.5678528
GGTATCA4650.033.48061
CGACGGT753.274E-431.3299927
ACGCAGA15000.029.7634947
GACTATA804.771895E-429.3789622
CGCAGAG15200.029.3718688
TACATGG17150.028.50532
GTACATG17500.027.4975721
GTGTACG700.00866901226.9584051
CGACGAA350.00883921526.85428252-53
TCGTAAC350.00883921526.85428252-53
ACATGGG17950.026.1810533
ACGACGG909.5132843E-426.1083286
Produced by FastQC (version 0.11.2)