FastQCFastQC Report
Sun 14 Apr 2019
SRR6576415_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576415_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences474979
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG12780.26906452706330175No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA9910.20864080306708296No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA9190.19348223816210822No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA8330.1753761745256106No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC7700.1621124302337577No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT7520.158322789007514No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA7140.15032243530766623No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG6720.14147993911309764No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA6570.13832190475789455No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC6460.1360060128974123No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG6290.13242690729484882No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG6060.1275845879502041No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG5710.12021584112139694No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC5580.11747887801355429No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT5540.11663673551883347No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA5250.11053120243210754No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC5190.1092679886900263No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG4910.10337299122698057No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC4890.10295191997962015No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG4780.1006360281191379No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA11850.040.1206321
GTACATG26050.037.94691
TACATGG26300.037.5426452
ACATGGG27150.036.540453
AACGCAG13450.035.6527866
TCAACGC13850.034.966234
ACCTAAG4650.034.4184041
GGCCTAA550.0026950434.234351
CAACGCA14300.034.1910865
ATCAACG14050.034.133843
CTAAGAC5300.033.7107963
CATGGGG14700.033.584074
GGTACCT4600.032.6941878
ATAGTAC600.004150891731.3451293
GGGTACC4950.030.3824797
GATAATA1602.1191227E-929.386062
CCTAAGA5450.029.332142
ATGGGGG5350.028.9985125
TAATAGT1951.8189894E-1128.9339664
CGCAGAG16700.028.7053038
Produced by FastQC (version 0.11.2)