FastQCFastQC Report
Sun 14 Apr 2019
SRR6576417_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576417_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences261261
Sequences flagged as poor quality0
Sequence length100
%GC49

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4820.18448983966225346No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAA301.3693397E-462.6652645
CATGGGT4300.033.882964
ATGGGTA2650.033.6973575
TTATAAG600.004147826731.3446272
ACCAGTA902.5799536E-531.3446272
ATGGGAT1351.3191311E-831.3326325
TATACTC600.00415560931.3326325
ATAGTAC1052.0576608E-631.332633
TACATGG16000.030.5610142
GTACATG16350.029.9068031
CTAGGAC953.741881E-529.6835443
ACATGGG16600.029.1619973
GGTACCT2750.029.0538948
GGGTACC3150.026.8565437
ATTACAC700.00882471926.856543
GTAATAC700.00882471926.856543
GTAAGAC700.00882471926.856543
TAGGACC1807.4433046E-926.1105254
GTACCTG3100.025.7736159
GTCTTGC2208.731149E-1125.6456051
Produced by FastQC (version 0.11.2)