FastQCFastQC Report
Sun 14 Apr 2019
SRR6576417_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576417_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences261261
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5070.19405881474846992No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3320.12707598914495466No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3250.12439667612081405No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2980.11406218302770026No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2920.11176562900700833No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG804.616595E-946.9983755
TATAAGC608.807164E-539.165312
ATGGGTA2300.038.824745
TCTACTC651.4107897E-436.1525963
ACATGGG13700.032.2470553
TACATGG13700.031.560952
GTATTAG600.00409007431.4345231
GCTCTAC902.5299276E-531.4345231
GTACATG14000.030.985461
CATGGGT3500.030.8846454
CATGGGG5800.030.7920364
GGTACCT2200.029.9080568
GCCTATA650.00604950329.0164831
GTATCAA8100.027.3596781
ATGGGGA3100.027.2893775
ATCAACG8800.027.2376943
AACGCAG8700.027.010566
GTATAAG700.00868604926.9438761
GTTAGAG1057.4017626E-526.8562153
TATAATC700.00882524626.8562153
Produced by FastQC (version 0.11.2)