FastQCFastQC Report
Sun 14 Apr 2019
SRR6576418_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576418_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences223855
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4410.1970025239552389No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2510.11212615309016997No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGCG608.816495E-539.1552165
ACACCGT500.001701542837.5890126
GTATCAA7550.031.1306381
AACGCAG8100.029.5839426
CATGGGG5350.028.9821824
CAACGCA8550.028.5764435
GTGTTGC1005.2990814E-528.2043571
TCAACGC8500.028.1917574
TACATGG12900.028.05862
GTACATG12900.027.6942021
ATACCGT856.8104616E-427.6389776
TACCGTC856.8104616E-427.6389777
ACATGGG13450.027.597883
ATCAACG8750.027.386283
ACGCAGA9000.026.625557
CGCAGAG9400.025.4925488
GCAGAGT9400.025.4925489
CATGGGT3000.025.0593414
ATGGGGA3000.025.0593415
TATCAAC9800.023.9832972
Produced by FastQC (version 0.11.2)