FastQCFastQC Report
Sun 14 Apr 2019
SRR6576418_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576418_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences223855
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5380.24033414487056354No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2970.13267516919434455No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA2610.11659333050412098No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2330.1040852337450582No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2250.10051149181389737No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGACG250.00521943656.430318
GCACCGT250.005233304656.3924946
CCCTTAG752.581146E-950.182711
GTATACC555.2735595E-542.721593
ACGGACC1006.9667294E-1042.322738
GTATCAA7050.039.372071
CGGACCA1101.7735147E-938.4752129
ATCAACG7450.035.9549453
AACGCAG7500.035.7152446
CAAGACG1204.1800376E-935.245314
TCAACGC7700.034.787584
CAACGCA7800.034.3415835
ATGGGTA1950.033.7390985
GTATAAG851.736759E-533.2091451
CATGGGT2550.033.172064
AAGACGG1309.129508E-932.5341345
ACGCAGA8350.032.0795637
CTTAGCC600.004156599831.3291653
CGCAGAG8750.030.6335968
GCGCAAG1401.8586434E-830.244041
Produced by FastQC (version 0.11.2)