FastQCFastQC Report
Sun 14 Apr 2019
SRR6576419_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576419_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences377068
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8080.21428495655955956No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4760.12623717737914647No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4290.11377258213372654No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4090.10846849905056911No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3970.10528604920067468No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3870.10263400765909596No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12550.035.633541
TACATGG24150.034.0616722
GTACATG24200.034.040961
ACATGGG24400.033.134753
ATGGGAT3450.031.3367375
CATGGGT5150.030.1197784
CTTATAG804.771825E-429.3781913
AACGCAG15050.029.0463456
CATGGGG9100.028.409684
TCAACGC15900.027.1979244
ATCAACG15950.027.1126633
CAACGCA16200.026.9844135
GTACTAA700.00876133726.8993051
CGAAATT350.00883516226.85649948-49
GTATAGC1257.908284E-626.3613171
CGCAGAG16600.026.3306948
GTAGCGC2156.548362E-1126.2354093
GTACTAC1458.507359E-725.9717431
ATGGGGC3100.025.7769955
ACGCAGA17050.025.6357487
Produced by FastQC (version 0.11.2)