FastQCFastQC Report
Sun 14 Apr 2019
SRR6576420_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576420_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences343315
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8970.2612760875580735No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4760.1386481802426343No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4630.13486157027802456No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4520.13165751569258552No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA3850.112141910490366No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA3770.10981168897368306No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA3500.10194719135487816No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3450.10049080290695134No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGT405.6791026E-446.98666
TACATGG25300.040.678122
GTACATG25750.039.967241
CATGGGT4850.039.720634
ACATGGG26350.038.8731653
ATGGGTA3400.038.6948475
GTATAAC550.00271622434.177051
CATGGGG9150.033.891974
ATGGGAG5100.033.167015
GGGTACC3300.031.32447
ATGGGAT2600.030.7220085
GTATCAA14450.030.5701311
CATGGGA14750.029.625454
GGTACCT3500.029.5344338
CTACTAG804.777881E-429.3709031
TAACGCA650.006156810628.9148314
TGGGTAC3700.027.9379796
ATATACC856.817641E-427.6391753
CTATTAA1205.8338683E-627.4128441
AACGCAG15800.027.3592876
Produced by FastQC (version 0.11.2)