FastQCFastQC Report
Sun 14 Apr 2019
SRR6576421_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576421_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences226214
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5320.23517554174365868No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3190.14101691318839682No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3180.140574853899405No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA2990.13217572740856004No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2720.12024012660578037No Hit
CCTCTAGGCGGTCACGGGCCTGGTTGCCCACTTCCTCCAGCCGCCCTCGG2620.11581953371586197No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT2420.10697834793602518No Hit
GGAGAAGGATACTCATTGATTCTCCTGGGCCACTGGGGTGATGATGGGGT2320.10255775504610677No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTACC200.002161071270.49718594
GTATCAA7550.042.9519231
GTATATA853.9381484E-738.7043341
AACGCAG9100.035.6359376
ATCAACG9300.035.3749283
GTACATG15250.035.1330221
TCAACGC9300.034.869574
TACATGG15300.034.711032
CAACGCA9450.034.316095
GGTTTAC550.002712950634.180451
ATAACGC702.1799929E-433.5700873
ACATGGG15600.033.4409683
TGTACAT600.004154797631.3320795
CATGGGA6750.030.635814
TATCAAC10850.030.321372
ACGCAGA10300.030.1153017
CATGGGT2550.029.4890174
ATGGGAG3400.029.028255
TTTGGCC650.006145064328.921923
GTCCGGC650.006145064328.9219294