FastQCFastQC Report
Sun 14 Apr 2019
SRR6576422_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576422_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences206435
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5280.2557705815389832No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3130.15162157579867755No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3100.15016833385811515No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2700.13079177465061642No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA2640.1278852907694916No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT2400.11625935524499237No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG2360.1143216993242425No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG2350.11383728534405503No Hit
CCTCTAGGCGGTCACGGGCCTGGTTGCCCACTTCCTCCAGCCGCCCTCGG2280.11044638748274274No Hit
GGCCTGTATCTTCTCCATCAGGTTTGCCCACTGGCGATGCATGTCTTCCA2170.10511783370068059No Hit
GGAGAAGGATACTCATTGATTCTCCTGGGCCACTGGGGTGATGATGGGGT2080.10075810787899338No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGTTC200.002162912270.4798363
ATGGGCG408.0242935E-658.7331925
GTACTAG250.005220850556.424871
CCCGCTA250.00523589956.3838653
CCCGCAA250.00523589956.3838653
TGCCCGC701.4060788E-953.7379761
CCGAACT405.6535285E-447.020731
GTACATG15350.039.8221131
TACATGG15600.039.183942
ACATGGG15400.038.4435463
GCGAGAT250.001703155937.5892492-93
GGTACCT1750.037.589248
ATGGGTA1650.037.019715
CATGGGT2700.036.5450974
CATGGGG6050.036.5019534
TCCTAAG550.002706010634.196892
CCTATAA550.002706010634.196891
CTATCCT550.002715677734.172044
GGGTACC2000.032.8905877
GTATCAA7600.032.1720771
Produced by FastQC (version 0.11.2)